FastQCFastQC Report
Fri 28 Sep 2018
Pcal_RNAseq_real_pass_trimmed.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenamePcal_RNAseq_real_pass_trimmed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8184
Sequences flagged as poor quality0
Sequence length18-3770
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTTTTTTTTTTTTTTTT140.1710654936461388No Hit
ACGTTTTTTTTTTTTTTTTTTTTT90.10997067448680353No Hit
ACGTTTTTTTTTTTTTTT90.10997067448680353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAG351.8189894E-12357.696751
ACGGTAT600.0347.760741
CGGTATG500.0292.119022
GCGGTAT159.906372E-4278.20861
ATTGTGC159.906372E-4278.20863
TAGCCAC307.793824E-8278.20865
TTTTCGG159.906372E-4278.20865
GTTGTAC950.0263.566043
CACTTCG401.0131771E-9260.820569
GGTATGC650.0256.807923
CTGTTGT900.0255.024551
TGTTGTA1000.0250.387742
GGTAGCC501.2732926E-11250.387743
ACATTGT351.6812919E-7238.46451
TTGTACT1350.0231.840484
CGGTAGC552.5465852E-11227.62522
ATGCTTC650.0224.706946
ACAGTAT200.00234404208.656451
CCACTTC403.2716162E-7208.656458
GTAGCCA403.2716162E-7208.656454
Produced by FastQC (version 0.11.5)