FastQCFastQC Report
Fri 28 Sep 2018
Pcal_RNAseq_real_pass.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenamePcal_RNAseq_real_pass.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394085
Sequences flagged as poor quality0
Sequence length49-6182
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT21100.0288.256681
ACGGTAC10700.0260.444921
ACGGTAG9400.0242.347471
CTGTTGT29050.0232.972371
GTTGTAC27650.0206.37343
GTTGTGC16000.0189.37813
TTGTACT58250.0187.94834
ACATTGT24650.0187.524721
TGTACTT69900.0182.885735
ACCGGTA7300.0178.755041
ACGGTGT10500.0172.72441
TGTTGTA35550.0165.489782
ACGGTGG8750.0164.298811
CATTGTA29000.0157.870682
ATTGTAC34450.0150.229693
ACGTTTT22700.0148.097211
GGTATGC28200.0145.879073
GGTAGCC11300.0144.837973
CGGTATG24550.0144.144352
GTACTTC88750.0143.0456
Produced by FastQC (version 0.11.5)