FastQCFastQC Report
Tue 7 Aug 2018
P.cal-RNA_2_val_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameP.cal-RNA_2_val_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91928601
Sequences flagged as poor quality0
Sequence length20-100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGTGGAGAGCTAACAATTTCACACAGGAAAGCAGTGGTATCAACGC2652360.2885239165121201No Hit
GTGGAGAGCTAACAATTTCACACAGGAAAGCAGTGGTATCAACGCAGAGT1419960.15446335357589092No Hit
GGTATCAACGCAGAGCGGCCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1123810.12224813472359924No Hit
GTATCAACGCAGAGCGGCCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT937460.10197696797322087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTGG735750.041.457993
GTTAAGT751050.040.9157561
AGTGGAG945500.033.5444265
AAGTGGA913750.033.4134374
TTAAGTG997350.031.119982
GGAGAGC1034050.029.7160388
GGTTAAG157250.028.9479371
GAGAGCT1090500.027.8389039
GTGGAGA1137400.026.9489426
TGGAGAG1142350.026.7500577
TGAGCTA415400.024.53108494
TTCCCAG660500.022.5606084
CAGTCAC678550.022.1710668
TTTCCCA695850.021.5159363
CCCAGTC735450.020.2793256
CCTGGGT117050.020.1713289
CCAGTCA760800.019.7506437
GTACTAG273500.019.0284211
AGCTAAC1130450.018.9613197
TCCCAGT792600.018.77775
Produced by FastQC (version 0.11.5)