FastQCFastQC Report
Tue 7 Aug 2018
P.cal-RNA_1_val_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameP.cal-RNA_1_val_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91928601
Sequences flagged as poor quality0
Sequence length20-101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGTGGAGAGCTAACAATTTCACACAGGAAAGCAGTGGTATCAACGC2838590.30878202965364393No Hit
GGTATCAACGCAGAGCGGCCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1612110.1753654447542392No Hit
GTGGAGAGCTAACAATTTCACACAGGAAAGCAGTGGTATCAACGCAGAGT1490330.16211820736834665No Hit
GTATCAACGCAGAGCGGCCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1321050.14370391647752803No Hit
TATCAACGCAGAGCGGCCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1007240.10956764152214174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAAGT767000.042.60971
TAAGTGG787650.041.4819373
AAGTGGA962000.033.8688774
AGTGGAG994200.033.7962345
TTAAGTG1036700.031.9410972
GGTTAAG165650.031.1966651
GGAGAGC1094050.029.8344678
GAGAGCT1144800.028.3201759
TGGAGAG1204200.026.9001837
GTGGAGA1238200.026.4458396
TTCCCAG721800.024.9403084
TTTCCCA761250.023.7532423
CAGTCAC766800.023.6476868
CCCAGTC822400.021.9097376
CCAGTCA844900.021.439927
ACACCCC379400.021.12550494-95
TCCCAGT880300.020.473865
CCTGGGT114100.019.7951769
AGCTAAC1179950.019.509677
GTTTTCC1022550.018.6170141
Produced by FastQC (version 0.11.5)