FastQCFastQC Report
Thu 21 Jun 2018
DrWojtek_FireAnt_S1_L005_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDrWojtek_FireAnt_S1_L005_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269953173
Sequences flagged as poor quality0
Sequence length1-150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCT56598862.096617697470072No Hit
AGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTT29682121.0995284726658872No Hit
GGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTA21067210.7804023848239783No Hit
ACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTA12477430.46220719917228015No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT8545140.3165415655255143No Hit
GTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAG7608900.2818599950295824No Hit
CCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAA6769150.2507527481442124No Hit
TAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGT6289930.23300078047239695No Hit
CTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAAC5076450.18804928068024598No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG3742870.13864886114896674No Hit
AGTTTTTCCCACATAGTTTTTCCCACATAGTTTTTCCCACATAGTTTTTC2756630.10211511757263175No Hit
CCCACATAGTTTTTCCCACATAGTTTTTCCCACATAGTTTTTCCCACATA2730000.10112865019000906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG1124200.040.7124441
GACGCTC1367200.032.5343977
ACGCTCT1413700.031.4037658
TTGTGGG947000.029.8602642
GCCTAAC1180450.029.2109531
CGACGCT1599450.028.1046166
ACACGAC1774000.025.8635083
CGTTAGG727200.024.4799331
TACACGA1995600.023.2059062
CATGTGG1274750.022.987231
ACGACGC2071250.022.2231185
AGTTAGG736950.022.0816611
CACGACG2104950.021.7936554
CGCTCTT2113450.021.2780829
ATTGTGG1166550.018.524141
TGTTAGG847450.018.4858991
CTTGTGG645150.018.1814481
ACCACAT2369350.018.0839481
TCTAACC1052000.015.9417392
TGGTTAG1045250.015.6574481
Produced by FastQC (version 0.11.5)