FastQCFastQC Report
Thu 21 Jun 2018
DrWojtek_FireAnt_S1_L005_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDrWojtek_FireAnt_S1_L005_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269953173
Sequences flagged as poor quality0
Sequence length1-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCT12500440.4630595692238816No Hit
AGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTT7499700.277814849021982No Hit
GGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTAGGTTA4723590.17497812481722524No Hit
ACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTAACCTA2967330.10992017493345041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGC614150.047.6321951
GGGACCT682650.045.935651
GAGACCC801600.044.194151
GGAACCC769300.043.984071
GTCTAGC857750.043.5785458
TGAGGCC762800.043.173741
TGCCCTA768950.043.1223561
TGGGCTG732450.042.9763571
TAGGCCG746100.042.4267541
TCCAGGC1049050.042.183498
ACCCAGG714850.040.971711
AGCCCTT844350.040.8022651
TCTGGGC831150.040.728398
GTAGGCC780200.040.0654871
TGACTAG730000.039.9762841
CCTAGCT814900.039.7287861
GGGTCTG791950.039.7029151
GAGGTCC850850.039.602391
CCTAGTC840350.039.49211
GCGCTAG773900.039.424291
Produced by FastQC (version 0.11.5)